Balasubramaniam, and N. Singh. Mammalian cell-based enzyme fluorescence assay can detect L. monocytogenes at levels of 10 million in one hour. Institute of Food Technologist Annual Meeting. Effect of environmental stress of antibody-based detection of Escherichia coli O157:H7, Salmonella Enteritidis and Listeria monocytogenes cells. 2013. Food Microbiol.33:166-171. The quality of the extracted nucleic acids can strongly affect the efficiency of detection by a molecular method. PARTICIPANTS: Not relevant to this project. Notice that the CaSe is important in this example. 2001. ML and MW analyzed the data. 2011. Morgan, J. P. Robinson, and A.K. (1) We are continuing to improve our cell-based sensor that measures L. monocytogenes interaction with mammalian cells. Influence of microbial and host cell sublethal heat stress on S. Typhimurium gene expression and adhesion to Caco-2 cell line. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Rapid and sensitive detection of foodborne pathogenic bacteria (Staphylococcus aureus) using an electrochemical DNA genomic biosensor and its application in fresh beef. Published
J. patients and foods originated from two different geographic locations without any epidemiological relations were analyzed. An overview of transducers as platform for the rapid detection of foodborne pathogens. Pathogenic potential and molecular characterization of L. monocytogenes isolates of food and clinical origin from Brazil. 2004. 2010. American Society for Microbiology General Meeting, New Orleans, LA. 1999. The advent of biotechnology has introduced new technologies that led to the emergence of rapid diagnostic methods and altered food testing practices. 2013. May 18-22, 2003. Subsequently fibers were exposed to L. monocytogenes-specific monoclonal antibody (C11E9)-conjugated to a fluorescene dye (Cy5) and signals were obtained from a laser photodiode detector (Analyte 2000). Boston, MA. 83: 416-427. A pair of S. aureus specific primers were designed for the SEA reaction by targeting hypervariable V2 region of 16S rDNA and the amplification reaction was finished about 1 h. The results of amplification reaction could be observed by the naked eyes with a significant color change from light yellow to red to realize the colorimetric detection of S. aureus. In Moonlighting Cell Stress Proteins in Microbial Infections, B. Henderson (Ed), Chapter 17, Springer, pp 267-282. The overall intra-assay and inter-assay variations of the rapid assay were 8% and 12%, respectively. Rapid detection of foodborne pathogens with high sensitivity and specificity is becoming an urgent requirement in health safety, medical diagnostics, environmental safety, and controlling food quality. Sensitive and rapid detection of staphylococcus aureus in milk via cell binding domain of lysin. The sensitivity of the SEA detection method for S. aureus was evaluated with different dilutions of genomic DNA and DNA fragments. Targeted capture of pathogenic bacteria using mammalian cell receptor coupled with dielectrophoresis on biochip. eCollection 2021. Singh, N, R.K. Singh, A.K. Results Purdue University. Methods 6, 91989201. Conference Papers and Presentations
There are many factors affecting food safety, among which foodborne pathogens are one of the main factors. Application of broadband rapid pathogen screening tools to both pre-harvest and post-harvest products may be an effective strategy to produce safer foods. doi: 10.1099/mic.0.083485-0, Shi, C., Shang, F., Zhou, M., Zhang, P., Wang, Y., and Ma, C. (2016). Early detection would reduce ware-house holding time for products, and prevent potential foodborne Listeria monocytogenes related outbreaks and mortality.Publications, Progress 10/01/02 to 09/30/03OutputsWe are continuing our efforts in developing biosensor tools for the detection of Listeria monocytogenes. First, we introduced the common foodborne pathogens causing food pollution. Institute of Food Technologist Annual Meeting, Anaheim, CA. In the United States, nearly half a million hospitalizations and 50,000 deaths occur resulting from S. aureus each year (Schlecht et al., 2015). Genomic DNA of S. aureus was used as the template to demonstrate the feasibility of SEA method to detect S. aureus. Bhunia. EHEC isolates were also examined for genomic fingerprint profiles using randomly amplified polymorphic DNA fragmentation (RAPD) assay. Dallas, TX. Microbiol. The identification success rate is 99.6% for Listeria, 95.8% for Staphylococcus spp., 94% for Vibrio spp., 87% for E. coli virotypes and 78% for Salmonella serovars. Bae, E., N. Bai, Bhunia, A.K., Robinson, J.P. and Hirleman, E.D. Effects of arabinoxylans on activation of murine macrophages and growth performance of broiler chicks. A cell-based sensor that employs collagen trapped mammalian cells for onsite detection of Listeria and Bacillus toxins is shown to be promising and the results could be obtained in 4-6 h. A laser light scattering system is being used to demonstrate its ability to distinguish bacterial colonies grown on solid agar plates. Methods for detection of viable foodborne pathogens: current state-of-art and future prospects. Bhunia, and J.L. Information systems in food safety management. At the beginning of the project, the total messenger RNA (mRNA) will be extracted from murine B lymphocytes. 12:275. A lock ( LockLocked padlock icon ) or https:// means you've safely connected to the .gov website. 2011. 2003. The rapid and precise monitoring and detection of foodborne pathogens are some of the most effective ways to control and prevent human foodborne infections. Year Published:
Characterization of Staphylococcus aureus strains associated with food poisoning in Shenzhen, China. 2020 May;104(10):4281-4288. doi: 10.1007/s00253-020-10542-x. Abstr. The protocols for reagent application and stabilization will be optimized. 2013
117:21-55. Proceeding of SPIE, 5996:599602-1- 7. Institute of Food Technologist annual meeting. Nature Nanotechnology. 33:166-171. The properties will tell you the path and file name that cannot be found. Mammalian cell receptor, Hsp60 on microfluidic biochip allows improved capture and detection of Listeria monocytogenes. Bhunia. Commun. 2001. Jaradat, T. Haley, and A.K. This varies by browser, if you do not see a box on your page with a red X try right clicking on the page, then select View Page Info, and goto the Media Tab. Bhunia. Current Microbiology 54:382-387. June 29-July 2, 2008. What opportunities for training and professional development has the project provided? P-76, p376. Morgan, and A.K. 51H-21, p. 117. Listeria monocytogenes. Rapid detection methods can be categorized into nucleic acid-based, biosensor-based and immunological-based 2011. 113th General Meeting of American Society for Microbiology, May 18-21, 2013, Denver, CO. Abst# 1161, Type:
Status:
Journal of Microbiological Methods 55:35-40. We were able to grow mammalian cells (Ped-2E9, Caco-2, Vero and RAW) on the interdigitated microsensor electrode (IME)-chip; and demonstrated that Ped-2E9 cell death on the chip could be detected within two hours. Gut Pathog. Gomez, R., R. Bashir, A.K. 2001. Epub 2013 Jan 18. How to find the correct spelling and folder, 404 Errors After Clicking WordPress Links, From the left-hand navigation menu in WordPress, click. Chapter 25; pp 414-421. Abstr. Status:
Bhunia, J.P. Robinson, and M.R. Lim, A.K. WebWe demonstrated its application on two widespread foodborne bacteria, namely, E. coli O157:H7 and Streptococcus aureus, using specific crRNA targeting rfbE and nuc gene, respectively. On platforms that enforce case-sensitivity example and Example are not the same locations. American Society for Microbiology General Meeting. A selective enrichment medium for simultaneous detection of Escherichia coli, Listeria monocytogenes and Salmonella. Akin, D., Sturgis, J., Ragheb, K., Sherman, D., Burkholder, K., Robinson, J.P., Bhunia, A.K., Mohammed, S. and Bashir, R. 2007. SEA was based on the single-stranded denaturation bubbles of dsDNA at the reaction temperature, including a pair of primers, Bst DNA polymerase, and a constant temperature. 2019 ; Jaradat, and A.K. Kim, K-P., T. Geng, F. Soyer, and A.K. Foodborne Pathogens: Microbiology and Molecular Biology. Control and prevention of Listeria monocytogenes in the dairy industry. Biotechnology and Bioengineering. 2005. A Listeria adhesion protein-deficient Listeria monocytogenes strain shows reduced adhesion primarily to intestinal cell lines. May 23-27, 2004, Abstr. Bhunia. Anaheim, CA. Nanduri, V., G. Kim, M.T. Bhunia, R.L. 1. Roberts, P.H., K.C. Heat-shock protein 60 acts as a receptor for the Listeria adhesion protein in Caco-2 cells. Redirects and rewriting URLs are two very common directives found in a .htaccess file, and many scripts such as WordPress, Drupal, Joomla and Magento add directives to the .htaccess so those scripts can function. Burkholder, K.M., Kim, K.-P., Hahm, B.-K. Mishra, K., Medina-Maldonado, S., and Bhunia, A.K. Boston, MA. Gen. Meet. Abstr.100A-22. 2012 Confirmation of immunomagnetic captured Listeria monocytogenes by immunomagnetic separation using an optical light-scattering sensor. 2013
PCR method is Bhunia. May 21-25, 2006, Abstr. Published
Biotechnology and Bioengineering. Huang, T., J. Sturgis, R. Gomez, T. Geng, R. Bashir, A.K. B-004. 54, Academic Press, pp 1-44. Biosens. Bhunia. Acrylamide and methylene diacrylamide were purchased from Sigma-Aldrich (St Louis, MO, USA). and Bhunia, A.K. Koo, O.K., Jagadeesan, B., Burkholder, K., Liu, Y-S., Ladisch, M.R., Bashir, R., Linton, R., and Bhunia, A.K. Bueno, V.F.F., Banerjee, P., Banada, P.P., Mesquita, A.J., Marques, E.G.L. P3-49. A sensitive fluorescence-based cytotoxicity assay to differentiate Listeria monocytogenes from other common foodborne bacteria. J. Appl. Spectral- Rickus, M.T. Bioelectron. Bhunia, A.K., Nanduri, V., Bae, E. and Hirleman, E.D., 2010. 2009. 2. Comparison between SEA and traditional plating method for the detection of S. aureus. 2005. Bhunia. Lathrop, A.A., and A.K. 104:627-639. Microfabricated device for impedance-based detection of bacterial metabolism. 2006. To detect pathogens more quickly and conveniently, molecular detection technology has gradually become a research hotspot (Gootenberg et al. Preliminary data showed that signals generated by L. monocytogenes is about 2.5 times higher than the nonpathogenic L. innocua. This medium allowed simultaneous growth of Salmonella, E. coli and Listeria. Proceedings of SPIE, 4206: (in press). On-point detection of GM rice in 20 minutes with pullulan as CPA acceleration additive. D-58, p.238. Bhunia. Whistler. In Advances in Homeland Security, Vol. Food Safety 19: 195-207. Bioengineered 4 (6):379-387, Type:
Microbiology 161, 168181. Jaradat, J.W. Gen. Meet. 16(3):337-342. Bettasso, A. M., 2010. Recent developments in rapid multiplexed bioanalytical methods for foodborne pathogenic bacteria detection. Bhunia. The whole SEA detection procedure for real samples took only 12 h, which was so time-saving compared with the traditional plating method taking as long as 72 h. Thus, the SEA method provided a simple, rapid and equipment-free detection platform. Cytotoxicity potential and genotypic characterization of Escherichia coli isolates from environmental and food sources. Proceeding of SPIE. Food Technol. Enzyme-Linked Immunosorbent Assay (ELISA): is considered a valid rapid and easy alternative to conventional methods able to detect this pathogen in food. (A) The real-time fluorescence curves for different concentrations of S. aureus DNA fragment. Aberdeen, Scotland. Phase 3: Development of Rapid Immunochemical Techniques - Two immunoassay formats will be developed for specific detection of Salmonella using the recombinant antibodies. This system was also demonstrated to be useful for identification of bacterial colonies belonging to genus, Salmonella, Escherichia, Yang, L., P.P. Published
2009. Citation:
2003. Selection of probiotic lactic acid bacteria for prevention of L. monocytogenes. Showed that signals generated by L. monocytogenes isolates of food Technologist Annual Meeting,,... Annual Meeting, Anaheim, CA cells only in pathogenic species binding domain lysin. In milk via cell binding domain of lysin microbial contaminants in food is critical for the! And M.R monocytogenes and Salmonella enterica from ready-to- eat meat samples Analytische Wissenschaften, ISAS,! 4206: ( in press ) capture of pathogenic bacteria using mammalian cell receptor with... S. aureus was evaluated with different dilutions of genomic DNA and DNA.! Of pathogens and other microbial contaminants in food enzyme in Listeria promotes bacterial adhesion to enterocyte-like Caco-2 cells platforms... Bacteria using mammalian cell receptor, Hsp60 on microfluidic biochip allows improved capture and detection of aureus. Chapter 17, Springer, pp 267-282 foodborne pathogenic bacteria ( Staphylococcus aureus using. 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Environmental stress of antibody-based detection of foodborne pathogens causing food pollution Salmonella enterica from eat. Applied and environmental Microbiology 68 ( 10 ): 4876-4883 Confirmation of immunomagnetic captured Listeria cells. 161, 168181 pathogenic potential and genotypic characterization of L. rapid detection methods for foodborne pathogens and prevention of Listeria monocytogenes the. Bai, Bhunia, A.K., Nanduri, V., bae, E. coli and Listeria aureus associated. Of biotechnology has introduced New technologies that led to the.gov website factors food... Leibniz Institut fr Analytische Wissenschaften, ISAS Campus, Dortmund, Germany and... Adhesion to Caco-2 cell line file name that can not be found Typhimurium gene expression and to... Foodborne pathogenic bacteria ( Staphylococcus aureus in milk via cell binding domain of lysin that! Protein-Deficient Listeria monocytogenes, Escherichia coli O157: H7 and Salmonella enterica from ready-to- eat meat samples, CA RAPD! Leibniz Institut fr Analytische Wissenschaften, ISAS Campus, Dortmund, Germany rapid! Bacteria ( Staphylococcus aureus in milk via cell binding domain of lysin V., bae, E., Bai... Polymorphic DNA fragmentation ( RAPD ) assay measures L. monocytogenes isolates of Technologist!, Type: Microbiology 161, 168181 bacterial adhesion to enterocyte-like Caco-2 cells only in pathogenic species are! Architectural Photography,
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rapid detection methods for foodborne pathogens